Rapid communication: nucleotide sequence of the promoter and first exon of the somatotropin receptor gene in cattle.

Accession Number. U15731. Species. Bos taurus. Origin of Clone. Hybridizing with a 220-bp cDNA of the somatotropin receptor (Hauser et al., 1990) 10 clones were identified from a bovine genomic library (EMBL3 SP6pT7 cloning vector; Clontech Laboratories, Palo Alto, CA). A 2.9-kb XhoIIAatII fragment containing the promoter and exon I was subcloned into pGEM-7Zf (Promega, Madison, WI), and both sense and antisense strands sequenced from a single clone using a Sequenase kit (Amersham Life Science, Arlington Heights, IL). Comparison with Related Sequences. Compared to the analogous ovine sequence (O'Mahoney et al., 19941, the bovine sequence (Figure 1) is 94% identical from -500 to -1 and 45% identical from -670 to -500. The sequence from -2734 to -670 was not reported for the ovine. The DNA sequence of the first exon (double underline; untranslated in the bovine) was 100% identical to the first exon of the bovine cDNA of Hauser et al. (1990), 96% identical to the sheep cDNA of Adams et al. (1990), 72% identical to the human variant 1 cDNA of Pekhletsky et al. (19921, and 64% identical to the rabbit cDNA of Leung et al. (1987). Sequence Data. Differences in the promoter sequences of the somatotropin receptor between sheep (O'Mahoney et al., 1994) and cattle, from -500 bp to the transcription start site (+ 1 ), include an additional nine nucleotides within the bovine sequence (-397 to -383, underlined) and an insertion of six guanosine residues ( V) within the ovine sequence. Other differences were found as single base changes. The bovine sequence contains a TATA box (bold) at -30 bp but does not contain a consensus sequence for a CCAAT box (found in ovine). Comments. Because of the high homology of bovine and ovine sequences within the -500 to -1 region, and in light of the functional analysis of the ovine promoter presented by OMahoney et al. (1994), it can bovine somatotropin receptor gene.